1
Further define MARCO and SR-AI/II function, and may also be useful to identify other novel scavenger-type macrophage receptors and for additional studies of particle toxicology.BackgroundThe pulmonary alveolar macrophage (AM) plays an important role in defense of the lung [1-5]. Class A scavenger receptors (SRA) primarily expressed on the macro-phage (M? surface are critical for binding, uptake, a
1
Ovided a very important tool to facilitate biological study of macrophages [20-23]. Several murine macrophage cell lines from bone marrow [24,25], spleen [26,27], fetal liver [28,29], and lung [30] have been successfully obtained by in vitro infection of primary cell cultures with a recombinant J2 retrovirus carrying the v-raf and v-myc oncogenes. In addition, investigation of the function of both
1
Cell lines from primary alveolar macrophages from MS-/- mice. Results: We used in vitro infection of the primary AMs with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Following initial isolation in media supplemented with murine macrophage colony-stimulating factor (M-CSF), we subcloned three AM cell lines, designated ZK-1, ZK-2 and ZK-6. These cell lines grow well in RPMI-1640-10 FB
1
Ovided a very important tool to facilitate biological study of macrophages [20-23]. Several murine macrophage cell lines from bone marrow [24,25], spleen [26,27], fetal liver [28,29], and lung [30] have been successfully obtained by in vitro infection of primary cell cultures with a recombinant J2 retrovirus carrying the v-raf and v-myc oncogenes. In addition, investigation of the function of both
1
One of the newly sequenced gag genes (BS72) was apparently derived through recombination between F2 and CRF36_cpx parental viruses, one of the nef genes was apparently derived through recombination between F and CRF22_01A1 parental viruses. The phylogenetic analysis of gag sequences derived from the Cameroonian samples further revealed four sequences (BS09, BS25, BS16 and BS42) situated on diverge
1
Bited a 325-bp PCR product, whereas SR-AI/II-/- mutant allele showed a 434-bp PCR product. MARCO wild type allele exhibited ca. 500-bp PCR product, and MARCO-/- mutant exhibited ca. 850-bp PCR product. All of the three cell lines are stable and Mycoplasma-free by Mycoplasma PCR ELISA test (Roche, Indianapolis, IN) during culture in the past 24 months.SR-AI/IIM WT ZK1 ZK2 ZK6 MMARCOWT ZK1 ZK2 ZKMAR
1
Ion of immortalized MARCO and SR-AI/II-deficient murine alveolar macrophage cell linesHongwei Zhou1, Amy Imrich1 and Lester Kobzik*1,Address: 1Department of Environmental Health, Harvard School of Public Health, Boston, MA, 02115, USA and 2Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA Email: Hongwei Zhou - hzhou@hsph.harvard.edu; Amy Imrich -
1
Eshly isolated AMs from MS-/- mice, the cell lines exhibit decreased phagocytosis of unopsonized titanium dioxide (TiO2), fluorescent latex beads and bacteria (Staphylococcus aureus) compared with the primary AMs from wild type (WT) C57BL/6 mice. Conclusion: Our results indicated that three contiguous murine alveolar macrophage cell lines with MS-/- (ZK1, ZK2 and ZK6) were established successfully