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Tion and analysis, decision to publish or preparation of the manuscript.Tongo et al. Virology Journal 2013, 10:29 http://www.virologyj.com/content/10/1/Page 7 of17. Montavon C, Vergne L, Bourgeois A, Mpoudi-Ngole E, Malonga-Mouellet G, Butel C, Toure-Kane C, Delaporte E, Peeters M: Identification of a new circulating recombinant form of HIV type 1, CRF11-cpx, involving subtypes A, G, J, and CRF01-
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R macrophages which was derived from brochoalveolar lavage (BAL) obtained from MS-/- mice [31]. Immortalization was conducted by infection of the primary AMs from MS/- mice with a retrovirus J2. The immortalized AMs were cloned by limiting dilution method. Three of the clones, designated as ZK-1, ZK-2 and ZK-6 were chosen for further characterization of macrophage phenotype and phagocytic function
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Onfim I, Camacho RJ, Vandamme AM, Lemey P: Phylodynamics of the HIV-1 CRF02_AG clade in Cameroon. Infect Genet Evol 2012, 12:453?60. 20. Zhang M, Foley B, Schultz AK, Macke JP, Bulla I, Stanke M, Morgenstern B, Korber B, Leitner T: The role of recombination in the emergence of a complex and dynamic HIV epidemic. Retrovirology 2010, 7:25. 21. Carr JK, Salminen MO, Albert J, Sanders-Buell E, Gotte D
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Allele primers, amplifies a 434 bp DNA fragment from SRA-deficient ZK1, ZK2 and ZK6 cells. With primers for MARCO wild-type allele, amplifies a 500 bp DNA fragment from WT mice; with primers for MARCO mutant allele, amplifies a 850 bp DNA fragment from ZK cells. ZK1, ZK2 and ZK6 clones exhibited both MARCO and SRA-I/II-deficient. PCR products, ca.10 l/each was resolved on a 1.5 agarose gel by gel